Field-Portable Immunoassay Instruments and Reagents to Measure Chelators and Mobile Forms of Uranium

Blake, Diane A.

Tulane University, New Orleans, LA

关键词: Freezers;    46 Instrumentation Related To Nuclear Science And Technology;    Antibodies;    Immunoassay;    Hybridomas;   

DOI  :  10.2172/896790
RP-ID  :  ERSD-1025388-2006
RP-ID  :  None
RP-ID  :  896790

美国|英语

来源: UNT Digital Library

【 摘 要 】

Progress Report Date: 01/23/06 (report delayed due to Hurricane Katrina) Report of results to date: The goals of this 3-year project are to: (1) update and successfully deploy our present immunosensors at DOE sites; (2) devise immunosensor-based assays for Pb(II), Hg(II), chelators, and/or Cr(III) in surface and groundwater; and (3) develop new technologies in antibody engineering that will enhance this immunosensor program. Note: Work on this project was temporarily disrupted when Hurricane Katrina shut down the University on August 29, 2005. While most of the reagents stored in our refrigerators and freezers were destroyed, all of our hybridoma cell lines were saved because they had been stored in liquid nitrogen. We set up new tissue culture reactors with the hybridomas that synthesize the anti-uranium antibodies, and are purifying new monoclonal antibodies from these culture supernatants. Both the in-line and the field-portable sensor were rescued from our labs in New Orleans in early October, and we continued experiments with these sensors in the temporary laboratory we set up in Hammond, LA at Southeastern Louisiana University.