Field-Portable Immunoassay Instruments and Reagents to Measure Chelators and Mobile Forms of Uranium

Blake, Diane A.

Tulane University, New Orleans, LA

关键词: Modifications;    Antibodies;    Immunoassay;    Uranium;    Monoclonal Antibodies;   

DOI  :  10.2172/893777
RP-ID  :  NABIR-1011983-2003
RP-ID  :  None
RP-ID  :  893777

美国|英语

来源: UNT Digital Library

【 摘 要 】

The goals for the 3-year project period are (1) to test and validate the present uranium sensor and develop protocols for its use at the NABIR Field Research Center; (2) to develop new reagents that will provide superior performance for the present hand-held immunosensor; and (3) to develop new antibodies that will permit this sensor to also measure other environmental contaminants (chromium, mercury, and/or DTPA). Sensor design modifications are underway via international collaborations. New reagents that will provide superior performance for the present hand-held immunosensor are being prepared and tested. New methods have been developed, to produce recombinant forms of metal-specific monoclonal antibodies for use with the sensor. Site-directed mutagenesis experiments are underway to determine the mechanisms of binding. Immunization experiments with sheep and rabbits to develop new recombinant forms of antibodies to metal-chelate complexes (chromium, mercury, and/or DTPA) have been initiated.