High speed DNA sequence analysis by matrix-assisted laser desorption mass spectrometry. Final report for period February 15, 1991 - February 14, 2001

Smith, Lloyd M.

University of Wisconsin--Madison

关键词: Desorption;    Fragmentation;    Dna;    Nucleic Acids;    59 Basic Biological Sciences;   

DOI  :  10.2172/798154
RP-ID  :  DOE/ER/61130-10
RP-ID  :  FG02-91ER61130
RP-ID  :  798154

美国|英语

来源: UNT Digital Library

【 摘 要 】

This grant had as its focus (i) to develop chemistry and enzymology to permit the enzymatic synthesis of 2' fluoro modified Sanger sequencing reactions, which would be resistant to fragmentation during MALDI process, (ii) to develop rapid MALDI analyses of DNA sequence polymorphisms using Peptide Nucleic Acid (PNA) DNA analogs in conjunction with solid phase chemistry (iii) to study the fundamental mechanisms occurring in the MALDI analysis of nucleic acids.