Pulse requirements for electron diffraction imaging of single biological molecules

Hau-Riege, S ; London, R ; Chapman, H

Lawrence Livermore National Laboratory

关键词: Orientation;    Diffraction;    59 Basic Biological Sciences;    Electron Beams;    74 Atomic And Molecular Physics;   

DOI  :  10.2172/15014820
RP-ID  :  UCRL-TR-207533
RP-ID  :  W-7405-ENG-48
RP-ID  :  15014820

美国|英语

来源: UNT Digital Library

【 摘 要 】

The pulse requirements for electron diffraction imaging of single biological molecules are calculated. We find that the electron fluence and pulse length requirements imposed by the damage limit and by the need to classify the diffraction patterns according to their angular orientation cannot be achieved with today's electron beam technology. A simple analytical model shows that the pulse requirements cannot be achieved due to beam broadening due to spacecharge effects.