Detection of BCR-ABL Fusion mRNA Using Reverse Transcriptase Loop-mediated Isothermal Amplification

Dugan, L C ; Hall, S ; Kohlgruber, A ; Urbin, S ; Torres, C ; Wilson, P

关键词: AMPLIFICATION;    DETECTION;    LEUKEMIA;    MYELOID LEUKEMIA;    PATIENTS;   

DOI  :  10.2172/1034502
RP-ID  :  LLNL-TR-520096
PID  :  OSTI ID: 1034502
Others  :  TRN: US201204%%46

学科分类：生物科学（综合）

美国|英语

来源: SciTech Connect

【 摘 要 】

RT-PCR is commonly used for the detection of Bcr-Abl fusion transcripts in patients diagnosed with chronic myelogenous leukemia, CML. Two fusion transcripts predominate in CML, Br-Abl e13a2 and e14a2. They have developed reverse transcriptase isothermal loop-mediated amplification (RT-LAMP) assays to detect these two fusion transcripts along with the normal Bcr transcript.