Prediction of glycosaminoglycan synthesis in intervertebral disc under mechanical loading

JOURNAL OF BIOMECHANICS

卷:49

Article

Gao, Xin¹  Zhu, Qiaoqiao²  Gu, Weiyong^1,2 

[1] Univ Miami, Dept Mech & Aerosp Engn, Coral Gables, FL 33124 USA

[2] Univ Miami, Dept Biomed Engn, Coral Gables, FL 33124 USA

关键词: Intervertebral disc;    Glycosaminoglycan synthesis;    Mechanical stimuli;    Mechanobiology;    Multiscale mathematical model;    Finite element analysis;   

DOI  :  10.1016/j.jbiomech.2016.05.028

来源: Elsevier

【 摘 要 】

The loss of glycosaminoglycan (GAG) content is a major biochemical change during intervertebral disc (IVD) degeneration. Abnormal mechanical loading is one of the major factors causing disc degeneration. In this study, a multiscale mathematical model was developed to quantify the effect of mechanical loading on GAG synthesis. This model was based on a recently developed cell volume dependent GAG synthesis theory that predicts the variation of GAG synthesis rate of a cell under the influence of mechanical stimuli, and the biphasic theory that describes the deformation of IVD under mechanical loading. The GAG synthesis (at the cell level) was coupled with the mechanical loading (at the tissue level) via a cell-matrix unit approach which established a relationship between the variation of cell dilatation and the local tissue dilatation. This multiscale mathematical model was used to predict the effect of static load (creep load) on GAG synthesis in bovine tail discs. The predicted results are in the range of experimental results. This model was also used to investigate the effect of static (0.2 MPa) and diurnal loads (0.1/0.3 MPa and 0.15/0.25 MPa in 12/12 hours shift with an average of 0.2 MPa over a cycle) on GAG synthesis. It was found that static load and diurnal loads have different effects on GAG synthesis in a diurnal cycle, and the diurnal load effects depend on the amplitude of the load. The model is important to understand the effect of mechanical loading at the tissue level on GAG synthesis at the cellular level, as well as to optimize the mechanical loading in growing engineered tissue. (C) 2016 Elsevier Ltd. All rights reserved.

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