Purification from human plasma of a tetrapeptide that potentiates insulin‐like growth factor‐I activity in chick embryo cartilage

FEBS Letters

Belleville, F.³  Heulin, M.H.³  Maachi, F.³  Le Nguyen, D.²  Becchi, M.¹  Nabet, P.³  Straczek, J.³ 

[1] Central Analysis Laboratory, C.N.R.S., Vernaison, France;Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 376, Centre Hospitalier Universitaire Arnaud de Villeneuve, Montpellier Cedex 05, France;Biochemistry Laboratory, Faculty of Medicine, University of Nancy I, B.P. 184, 54505 Vandoeuvre Cedex, France

关键词: Glycosaminoglycan synthesis;    Insulin-like growth factor I;    Peptide purification;    Wound repair;    Human plasma;    Chick embryo chondrocyte;    IGF;    insulin-like growth factor;    Da;    Daltons;    rh;    recombinant human;    MW;    molecular weight;    RP HPLC;    reverse phase high-performance liquid chromatography;    Boc;    t-Butyloxycarbonyl;    BOP;    Castro's reagent;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/0014-5793(95)01037-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human plasma has been shown to contain a low molecular weight factor that potentiates human IGF-I stimulation of glycosaminoglycan synthesis in chick embryo cartilage. The peptide was purified and characterized by Edman degradation and electrospray mass spectrometry. The primary structure determined was: Trp-Gly-His-Glu. A homologous synthetic peptide similarly promoted matrix biosynthesis in cartilage exposed to IGF-1.

【 授权许可】

Unknown