A simple and rapid procedure for the purification of synthetic polypeptides by a combination of affinity chromatography and methionine chemistry

FEBS Letters

Roggero, M.A.¹  Servis, C.¹  Corradin, G.¹ 

[1] Institute of Biochemistry, University of Lausanne, ch. des Boveresses 155, 1066 Epalinges, Switzerland

关键词: Peptide synthesis;    Peptide purification;    Affinity chromatography;    IMAC;    immobilized metal ion affinity chromatography;    P. berghei;    Plasmodium berghei;    TFA;    trifluoroacetic acid;    DIPC;    diisopropylcarbodiimide;    HOBT;    hydroxybenzotriazole;   

DOI  :  10.1016/S0014-5793(97)00441-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chemical synthesis of bioactive peptides has become a widespread and rapidly growing technique due to automated and efficient protocols for chain assembly. For most applications, the crude synthetic product must be purified to remove residual reactants, failure sequences and chemically modified peptide species. We propose here a method of universal applicability based on immobilized metal ion affinity chromatography, CNBr cleavage and use of reversible Met-sulfoxide protection. With this method we were able to purify to homogeneity in high yield the PbCS 242–310 polypeptide corresponding to the C-terminal region of Plasmodium berghei CS protein.

【 授权许可】

Unknown