Preservation of biomaterials and cells by freeze-drying: Change of paradigm

JOURNAL OF CONTROLLED RELEASE

卷:336

Review

Merivaara, Arto¹  Zini, Jacopo¹  Koivunotko, Elle¹  Valkonen, Sami¹  Korhonen, Ossi²  Fernandes, Francisco M.³  Yliperttula, Marjo¹ 

[1] Univ Helsinki, Fac Pharm, Div Pharmaceut Biosci, POB 56, Helsinki 00014, Finland

[2] Univ Eastern Finland, Sch Pharm, Kuopio 70210, Finland

[3] Sorbonne Univ, Fac Sci, Lab Chim Matiere Condensee Paris, UMR7574, F-75005 Paris, France

关键词: Freeze-drying;    Cells;    Extracellular vesicles;    Process analytical technology;    Quality-by-design;    Biophotonics;   

DOI  :  10.1016/j.jconrel.2021.06.042

来源: Elsevier

【 摘 要 】

Freeze-drying is the most widespread method to preserve protein drugs and vaccines in a dry form facilitating their storage and transportation without the laborious and expensive cold chain. Extending this method for the preservation of natural biomaterials and cells in a dry form would provide similar benefits, but most results in the domain are still below expectations. In this review, rather than consider freeze-drying as a traditional black box we break it through a detailed process thinking approach. We discuss freeze-drying from process thinking aspects, introduce the chemical, physical, and mechanical environments important in this process, and present advanced biophotonic process analytical technology. In the end, we review the state of the art in the freezedrying of the biomaterials, extracellular vesicles, and cells. We suggest that the rational design of the experiment and implementation of advanced biophotonic tools are required to successfully preserve the natural biomaterials and cells by freeze-drying. We discuss this change of paradigm with existing literature and elaborate on our perspective based on our new unpublished results.

【 授权许可】

Free