Inline monitoring of high cell density cultivation of Scenedesmus rubescens in a mesh ultra-thin layer photobioreactor by photon density wave spectroscopy

BMC Research Notes

Lena Bressel¹  Oliver Reich¹  Marvin Münzberg¹  Roland Hass²  Michael Sandmann³ 

[1] Physical Chemistry-innoFSPEC Potsdam, Institute of Chemistry, University of Potsdam, Am Mühlenberg 3, 14476, Potsdam, Germany;Physical Chemistry-innoFSPEC Potsdam, Institute of Chemistry, University of Potsdam, Am Mühlenberg 3, 14476, Potsdam, Germany;PDW Analytics GmbH, Geiselbergstr. 4, 14476, Potsdam, Germany;University of Applied Sciences Neubrandenburg, Brodaer Straße 2, 17033, Neubrandenburg, Germany;

关键词: Photon density wave spectroscopy;    Multiple light scattering;    Process analytical technology;    Fiber-optical spectroscopy;    Mesh ultra-thin layer photobioreactor;   

DOI  :  10.1186/s13104-022-05943-2

来源: Springer

【 摘 要 】

ObjectiveDue to multiple light scattering that occurs inside and between cells, quantitative optical spectroscopy in turbid biological suspensions is still a major challenge. This includes also optical inline determination of biomass in bioprocessing. Photon Density Wave (PDW) spectroscopy, a technique based on multiple light scattering, enables the independent and absolute determination of optical key parameters of concentrated cell suspensions, which allow to determine biomass during cultivation.ResultsA unique reactor type, called “mesh ultra-thin layer photobioreactor” was used to create a highly concentrated algal suspension. PDW spectroscopy measurements were carried out continuously in the reactor without any need of sampling or sample preparation, over 3 weeks, and with 10-min time resolution. Conventional dry matter content and coulter counter measurements have been employed as established offline reference analysis. The PBR allowed peak cell dry weight (CDW) of 33.4 g L−1. It is shown that the reduced scattering coefficient determined by PDW spectroscopy is strongly correlated with the biomass concentration in suspension and is thus suitable for process understanding. The reactor in combination with the fiber-optical measurement approach will lead to a better process management.

【 授权许可】

CC BY