Enhancement of surface ligand display on PLGA nanoparticles with amphiphilic ligand conjugates

JOURNAL OF CONTROLLED RELEASE

卷:156

Article

Park, Jason¹  Mattessich, Thomas¹  Jay, Steven M.^1,3  Agawu, Atu¹  Saltzman, W. Mark^1,2  Fahmy, Tarek M.^1,2 

[1] Yale Univ, Dept Biomed Engn, New Haven, CT 06511 USA

[2] Yale Univ, Dept Chem Engn, New Haven, CT 06511 USA

[3] Brigham & Womens Hosp, Div Cardiovasc, Dept Med, Cambridge, MA 02139 USA

关键词: PLGA;    Nanoparticle;    Modification;    Targeted;    Drug delivery;    T cells;   

DOI  :  10.1016/j.jconrel.2011.06.025

来源: Elsevier

【 摘 要 】

Biodegradable polymeric nanoparticles are widely recognized as efficacious drug delivery vehicles, yet the rational engineering of nanoparticle surfaces in order to improve biodistribution, reduce clearance, and/or improve targeting remains a significant challenge. We have previously demonstrated that an amphiphilic conjugate of avidin and palmitic acid can be used to modify poly(lactic-co-glycolic acid) (PLGA) particle surfaces to display functional avidin groups, allowing for the facile attachment of biotinylated ligands for targeting or steric stabilization. Here, we hypothesized that the incorporation, density, and stability of surface-presented avidin could be modulated through varying the lipophilicity of its fatty acid conjugate partner. We tested this hypothesis by generating a set of novel conjugates incorporating avidin and common fatty acids. We found that conjugation to linoleic acid resulted in a similar to 60% increase in the incorporation of avidin on the nanoparticle surface compared to avidin-palmitic acid, which exhibited the highest avidin incorporation in previous studies. Further, the linoleic acid-avidin conjugate yielded nanoparticles with enhanced ability to bind biotinylated ligands compared to the previous method; nanoparticles modified with avidin-linoleic acid bound similar to 170% more biotin-HRP than those made with avidin-palmitic acid and similar to 1300% more than particles made without conjugated avidin. Most critically, increased ligand density on anti-CD4-targeted nanoparticles formulated with the linoleic acid-avidin conjugate resulted in a 5% increase in binding of CD4(+) T cells. Thus we conclude that the novel avidin-linoleic acid conjugate facilitates enhanced ligand density on PLGA nanoparticles, resulting in functional enhancement of cellular targeting. (C) 2011 Elsevier B.V. All rights reserved.

【 授权许可】

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