In vitro and in vivo evaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineering

JOURNAL OF CONTROLLED RELEASE

卷:205

Article; Proceedings Paper

Vo, Tiffany N.¹  Ekenseair, Adam K.¹  Spicer, Patrick P.¹  Watson, Brendan M.¹  Tzouanas, Stephanie N.¹  Roh, Terrence T.¹  Mikos, Antonios G.^1,2 

[1] Rice Univ, Dept Bioengn, Houston, TX 77251 USA

[2] Rice Univ, Dept Chem & Biomol Engn, Houston, TX 77251 USA

关键词: Thermogelling;    Tissue engineering;    Bone;    Poly(N-isopropylacrylamide);   

DOI  :  10.1016/j.jconrel.2014.11.028

来源: Elsevier

【 摘 要 】

In this study, we investigated the mineralization capacity and biocompatibility of injectable, dual- gelling hydrogels in a rat cranial defect as a function of hydrogel hydrophobicity from either the copolymerization of a hydrolyzable lactone ring or the hydrogel polymer content. The hydrogel system comprised a poly( N-isopropylacrylamide)- based thermogelling macromer ( TGM) and a polyamidoamine crosslinker. The thermogelling macromer was copolymerized with ( TGM/ DBA) or without ( TGM) a dimethyl-gamma-butyrolactone acrylate (DBA)-containing lactone ring that modulated the lower critical solution temperature and thus, the hydrogel hydrophobicity, over time. Three hydrogel groups were examined: (1) 15 wt.% TGM, (2) 15 wt.% TGM/ DBA, and (3) 20 wt.% TGM/DBA. The hydrogels were implanted within an 8 mm critical size rat cranial defect for 4 and 12 weeks. Implants were harvested at each timepoint and analyzed for bone formation, hydrogel mineralization and tissue response using microcomputed tomography(microCT). Histology and fibrous capsule scoring showed a light inflammatory response at 4 weeks that was mitigated by 12 weeks for all groups. MicroCT scoring and bone volume quantification demonstrated a similar bone formation at 4 weeks that was significantly increased for the more hydrophobic hydrogel formulations -15 wt.% TGM and 20wt.% TGM/DBA -from4weeks to 12 weeks. A complementary in vitro acellular mineralization study revealed that the hydrogels exhibited calcium binding properties in the presence of serum-containing media, which was modulated by the hydrogel hydrophobicity. The tailored mineralization capacity of these injectable, dual-gelling hydrogels with hydrolysisdependent hydrophobicity presents an exciting property for their use in bone tissue engineering applications. (C) 2014 Elsevier B.V. All rights reserved.

【 授权许可】

Free