Controlled enzyme-catalyzed degradation of polymeric capsules templated on CaCO3: Influence of the number of LbL layers, conditions of degradation, and disassembly of multicompartments

JOURNAL OF CONTROLLED RELEASE

卷:162

Article

Marchenko, Irina⁶  Yashchenok, Alexey^4,5  Borodina, Tatiana⁶  Bukreeva, Tatiana⁶  Konrad, Manfred³  Mohwald, Helmuth⁴  Skirtach, Andre^1,2,4 

[1] Univ Ghent, Dept Mol Biotechnol, B-9000 Ghent, Belgium

[2] Univ Ghent, NB Photon, B-9000 Ghent, Belgium

[3] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany

[4] Max Planck Inst Colloids & Interfaces, D-14424 Potsdam, Germany

[5] Saratov NG Chernyshevskii State Univ, Saratov 410012, Russia

[6] Russian Acad Sci, Inst Crystallog, Moscow 119333, Russia

关键词: Microcapsules;    Layer-by-layer;    Drug delivery;    Calcium carbonate;    Controlled release;   

DOI  :  10.1016/j.jconrel.2012.08.006

来源: Elsevier

【 摘 要 】

Enzyme-catalyzed degradation of CaCO3-templated capsules is presented. We investigate a) biodegradable, b) mixed biodegradable/synthetic, and c) multicompartment polyelectrolyte multilayer capsules with different numbers of polymer layers. Using confocal laser scanning microscopy we observed the kinetics of the non-specific protease Pronase-induced degradation of capsules is slowed down on the order of hours by either increasing the number of layers in the wall of biodegradable capsules, or by inserting synthetic polyelectrolyte multilayers into the shell comprised of biodegradable polymers. The degradation rate increases with the concentration of Pronase. Controlled detachment of subcompartments of multicompartment capsules, with potential for intracellular delivery or in-vivo applications, is also shown. (C) 2012 Elsevier B. V. All rights reserved.

【 授权许可】

Free