Epigallocatechin gallate induces apoptosis of monocytes

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:115

Article

Kawai, K ; Tsuno, NH ; Kitayama, J ; Okaji, Y ; Yazawa, K ; Asakage, M ; Sasaki, S ; Watanabe, T ; Takahashi, K ; Nagawa, H

关键词: monocyte;    apoptosis;    catechin;    EGCG;    inflammation;   

DOI  :  10.1016/j.jaci.2004.10.005

来源: Elsevier

【 摘 要 】

Background: Monocytes are the main effector cells of the immune system, and the regulation of their survival and apoptosis is essential for monocyte-involved immune responses. Green tea polyphenol catechin has been reported to have antiallergic and antiinflammatory activities, but its effect on monocytes has not yet been explored. Objective: To elucidate the mechanisms of the anti-inflammatory effect of catechin, we studied the effect of catechin, especially epigallocatechin gallate (EGCG), on the apoptosis of monocytes. Methods: Isolated peripheral blood monocytes were incubated without or with catechin, and apoptosis was evaluated by annexin V and propidium iodide double-staining or terminal deoxynucleotidyl assay. The activation of caspases 3, 8, and 9 was also evaluated by flow cytometry. The influence of GMCSF or LPS, the known monocyte survival factors, on the EGCG-induced apoptosis of monocytes was investigated. Results: Among the 4 catechin derivatives tested, EGCG and epicatechin gallate induced apoptosis of monocytes. Caspases 3, 8, and 9, which play a central role in the apoptotic cascade, were dose-dependently activated by EGCG treatment. The EGCG-induced apoptosis of monocytes was not affected by GM-CSF or LPS. Conclusion: Catechin, especially EGCG, by promoting monocytic apoptosis, may be a new promising antiinflammatory agent, and should be tested in clinical trials.

【 授权许可】

Free