Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro

International Journal of Molecular Sciences

Koji Kaida³  Yoshitomo Honda¹  Yoshiya Hashimoto²  Masahiro Tanaka⁴  Shunsuke Baba³ 

[1] Institute of Dental Research, Osaka Dental University, Osaka 573-1121, JapanDepartment of Biomaterials, Osaka Dental University, Osaka 573-1121, Japan;Department of Oral Implantology, Osaka Dental University, Osaka 573-1121, Japan;Department of Fixed Prosthodontics and Occlusion, Osaka Dental University, Osaka 573-1121, Japan;

关键词: polyphenol;    catechin;    EGCG;    bone;    dedifferentiated fat cell;    stem cells;    osteoblast;   

DOI  :  10.3390/ijms161226081

来源: mdpi

【 摘 要 】

Despite advances in stem cell biology, there are few effective techniques to promote the osteogenic differentiation of human primary dedifferentiated fat (DFAT) cells. We attempted to investigate whether epigallocatechin-3-gallate (EGCG), the main component of green tea catechin, facilitates early osteogenic differentiation and mineralization on DFAT cells in vitro. DFAT cells were treated with EGCG (1.25–10 μM) in osteogenic medium (OM) with or without 100 nM dexamethasone (Dex) for 12 days (hereafter two osteogenic media were designated as OM(Dex) and OM). Supplementation of 1.25 μM EGCG to both the media effectively increased the mRNA expression of collagen 1 (COL1A1) and runt-related transcription factor 2 (RUNX2) and also increased proliferation and mineralization. Compared to OM(Dex) with EGCG, OM with EGCG induced earlier expression for COL1A1 and RUNX2 at day 1 and higher mineralization level at day 12. OM(Dex) with 10 μM EGCG remarkably hampered the proliferation of the DFAT cells. These results suggest that OM(without Dex) with EGCG might be a preferable medium to promote proliferation and to induce osteoblast differentiation of DFAT cells. Our findings provide an insight for the combinatory use of EGCG and DFAT cells for bone regeneration and stem cell-based therapy.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.