Tape strips detect distinct immune and barrier profiles in atopic dermatitis and psoriasis

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:147

Article

He, Helen^1,2  Bissonnette, Robert³  Wu, Jianni^1,2,4  Diaz, Aisleen^1,5  Proulx, Etienne Saint-Cyr³  Maari, Catherine³  Jack, Carolyn³  Louis, Maudeline³  Estrada, Yeriel¹  Krueger, James G.²  Zhang, Ning¹  Pavel, Ana B.¹  Guttman-Yassky, Emma^1,2 

[1] Icahn Sch Med Mt Sinai, Dept Dermatol, New York, NY 10029 USA

[2] Rockefeller Univ, Lab Invest Dermatol, New York, NY USA

[3] Innovaderm Res, Montreal, PQ, Canada

[4] Suny Downstate Med Ctr, Coll Med, New York, NY USA

[5] Ponce Hlth Sci Univ, Sch Med, Ponce, PR USA

关键词: Atopic dermatitis;    psoriasis;    tape strips;    biomarker;    skin;    RNA sequencing;    T(H)1;    T(H)2;    T(H)17;    immune;    epidermal barrier;   

DOI  :  10.1016/j.jaci.2020.05.048

来源: Elsevier

【 摘 要 】

Background: Our current understanding of atopic dermatitis (AD) and psoriasis pathophysiology is largely derived from skin biopsy studies that cause scarring and may be impractical in large-scale clinical trials. Although tape strips show promise as a minimally invasive technique in these common diseases, a comprehensive molecular profiling characterizing and differentiating the 2 diseases in tape strips is unavailable. Objective: Our aim was to construct a global transcriptome of tape strips from lesional and nonlesional skin of adults with moderate-to-severe AD and psoriasis. Methods: A total of 20 tape strips were obtained from lesional and nonlesional skin of patients with AD and psoriasis and skin from controls (n = 20 each); the strips were subjected to RNA sequencing (RNA-seq), with quantitative RT-PCR validation of immune and barrier biomarkers. Results: We detected RNA-seq profiles in 96 of 100 of samples (96%), with 4123 and 5390 genes differentially expressed in AD and psoriasis lesions versus in controls, respectively (fold change >= 2; false discovery rate [FDR] < 0.05). Nonlesional tape stripped skin from patients with AD was more similar to lesional skin than to nonlesional skin of patients with psoriasis, which showed larger differentiation from lesions. AD and psoriasis tissues shared increases in levels of dendritic cell and T-cell markers (CD3, ITGAX/CD11c, and CD83), but AD tissues showed preferential T(H)2 skewing (IL-13, CCL17/TARC, and CCL18), whereas psoriasis was characterized by higher levels of expression of T(H)17-related (IL-17A/F and IL-36A/IL-36G), T(H)1-related (IFN-gamma and CXCL9/CXCL10), and innate immunity-related (nitric oxide synthase 2/inducible nitric oxide synthase and IL-17C) products (FDR < 0.05). Terminal differentiation (FLG2 and LCE5A), tight junction (CLDN8), and lipid biosynthesis and metabolism (FA2H and ALOXE3) products were significantly downregulated in both AD and psoriasis (FDR < 0.05). Nitric oxide synthase 2/inducible nitric oxide synthase expression (determined by quantitative PCR) differentiated AD and psoriasis with 100% accuracy. Conclusion: RNA-seq tape strip profiling detected distinct immune and barrier signatures in lesional and nonlesional AD and psoriasis skin, suggesting their utility as a minimally invasive alternative to biopsies for detecting disease biomarkers.

【 授权许可】

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