Mast cells regulate IFN-γ expression in the skin and circulating IgE levels in allergen-induced skin inflammation

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:109

Article

Alenius, H ; Laouini, D ; Woodward, A ; Mizoguchi, E ; Bhan, AK ; Castigli, E ; Oettgen, HC ; Geha, RS

关键词: atopic dermatitis;    mast cells;    inflammation;    antibodies;    cytokines;    in vivo animal models;   

DOI  :  10.1067/mai.2002.120553

来源: Elsevier

【 摘 要 】

Background: Mast cells are important effector cells in IgE-mediated allergic reactions. They are present in normal skin and increased in skin lesions of patients with atopic dermatitis (AD). Objective: We used mice deficient in mast cells (W/W-v) to assess, the role of these cells in a marine model of allergen-induced skin inflammation induced by repeated epicutaneous sensitization with ovalbumin (OVA); the model exhibits many of the characteristics of AD. Methods: Mice deficient in mast cells were sensitized with OVA. Histologic and immunohistochemical examinations, as well as measurements of IL-4 and IFN-gamma mRNA, were performed on OVA-sensitized skin. Total and antigen-specific serum IgE levels were determined. Results. Infiltration in W/W-v mice by mononuclear cells, T cells, and eosinophils in OVA-sensitized skin was comparable to that in wild-type (WT) controls. Expression of IL-4 mRNA in sensitized skin sites was similarly increased in WT and W/W-v mice. However. IFN-gamma mRNA expression was significantly increased in sensitized skin of W/W-v mice but not in that of WT controls. IL-4 mRNA was readily detectable in unsensitized skin of WT controls but not in that of W/W-v mice, whereas expression of IL-12 p40 mRNA was significantly increased in unsensitized skin of W/W-v mice in comparison with WT controls. Total serum IgE levels were. significantly increased after epicutancous sensitization in W/W-v mice in comparison with WT controls. Conclusion: These results suggest that mast cells regulate IFN-gamma expression in the skin and IgE levels in the circulation in a model of allergen-induced skin inflammation with similarities to AD. This is important, given the role of IFN-gamma in keratinocyte injury in AD and the role of IgE-mediated reactions in exacerbating AD.

【 授权许可】

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