Serum IgE response to orally ingested antigen: A novel IgE response model with allergen-specific T-cell receptor transgenic mice

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:105

Article

Shida, K ; Hachimura, S ; Ametani, A ; Ishimori, M ; Ling, M ; Hashiguchi, M ; Ueda, Y ; Sato, T ; Kumagai, Y ; Takamizawa, K ; Habu, S ; Kaminogawa, S

关键词: IgE;    food allergy;    TCR transgenic mouse;    ovalbumin;    oral tolerance;    T-H2;    antibody response to orally ingested antigen;    animal model;   

DOI  :  10.1067/mai.2000.104934

来源: Elsevier

【 摘 要 】

Background: The mechanism by which orally ingested allergens elicit an IgE response remains unclear because there are few animal models available for investigation of this response. Objective: We tried to develop a murine model suitable for investigation of the IgE response to orally ingested allergens, which would allow us to identify T cells that could promote IgE production. Methods: Ovalbumin (OVA)-specific T-cell receptor transgenic mice were fed a diet containing OVA, and both the serum antibody response and cytokine production by splenocytes were examined. Results: Oral administration of OVA to transgenic mice Led to an increase in the levels of both antigen-specific IgE and total IgE in the sera. Subsequent intravenous challenge of OVA-fed transgenic mice with OVA resulted in anaphylactic shock. Analysis of cytokine production by splenocytes revealed that high IL-4-producing T cells appeared in the spleen 1 week after the start of feeding the OVA diet. T cells from these mice were found to promote IgE secretion by BALB/c B cells in vitro. This helper activity and the levels of IL-4 secretion were diminished after long-term feeding. These findings suggest the possibility that the orally ingested antigen elicited a response by a subpopulation of T cells that produce high levels of T-H2-type cytokines and that promote IgE secretion, and these same T cells were tolerized by the orally ingested antigen. Conclusion: This experimental model with transgenic mice may be a useful tool for further studies of the cellular and molecular mechanisms of the T-cell and IgE responses to orally ingested antigens.

【 授权许可】

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