Significance of ovomucoid- and ovalbumin-specific IgE/IgG4 ratios in egg allergy

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:129

Article

Caubet, Jean Christoph¹  Bencharitiwong, Ramon¹  Moshier, Erin¹  Godbold, James H.¹  Sampson, Hugh A.¹  Nowak-Wegrzyn, Anna¹ 

[1] Mt Sinai Sch Med, Dept Pediat, Div Allergy & Immunol, Jaffe Food Allergy Inst, New York, NY 10028 USA

关键词: Egg;    egg white;    ovalbumin;    ovomucoid;    egg allergy;    food allergy;    children;    hypersensitivity;    IgE;    IgG(4);    IgE/IgG(4) ratio;   

DOI  :  10.1016/j.jaci.2011.11.053

来源: Elsevier

【 摘 要 】

Background: The role of specific IgG(4) antibodies in natural tolerance acquisition remains a matter of debate; the specific IgE/IgG(4) ratio might add value to the measurement of absolute amounts of IgE for assessing the ongoing status of egg reactivity. Objective: We sought to determine the significance of IgG(4) antibodies to ovalbumin (OVA) and ovomucoid (OVM) in egg-allergic children. Methods: One hundred seven egg-allergic children (mean age 6.9 years; range 1.6-18.6 years) were challenged to baked egg. The outcomes of the challenges were related to the level of specific IgE and IgG(4) to OVM and OVA, component IgE/IgG(4) ratios, and mediator release in a functional assay based on the rat basophil leukemia cell line. Results: Baked egg-reactive children had significantly higher OVA and OVM ratios of IgE/IgG(4) and mediator release in the rat basophil leukemia-based assay than did tolerant children (P < .05 for both). The OVA- and OVM-specific IgE/IgG(4) ratios and mediator release were correlated. In the receiver operating characteristic analysis, the areas under the curve for a logistic regression model including specific IgE and IgG(4) to OVA and OVM were significantly greater compared with the areas under the curve for egg white-specific IgE and OVM-specific IgE. Conclusions: The balance between IgE and IgG(4) to OVA and OVM has functional consequences. A model that includes the interactions between IgE and IgG(4) to OVA and OVM accurately predicts reactivity to baked egg and warrants further investigation. (J Allergy Clin Immunol 2012; 129: 739-47.)

【 授权许可】

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