Unraveling heterogeneity in pediatric atopic dermatitis: Identification of serum biomarker based patient clusters

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

卷:149

Article

Bakker, Daphne S.^1,2  de Graaf, Marlies¹  Nierkens, Stefan²  Delemarre, Eveline M.²  Knol, Edward²  van Wijk, Femke²  de Bruin-Weller, Marjolein S.¹  Drylewicz, Julia²  Thijs, Judith L.¹ 

[1] Univ Med Ctr Utrecht, Natl Expertise Ctr Atop Dermatitis, Dept Dermatol & Allergol, Utrecht, Netherlands

[2] Univ Med Ctr Utrecht, Ctr Translat Immunol, Utrecht, Netherlands

关键词: Atopic dermatitis;    pediatric;    biomarkers;    endotypes;    personalized medicine;    principal components analysis;    cluster analysis;   

DOI  :  10.1016/j.jaci.2021.06.029

来源: Elsevier

【 摘 要 】

Background: Increasing evidence shows that pediatric atopic dermatitis (AD) differs from adult AD on a biologic level. Broad biomarker profiling across a wide range of ages of pediatric patients with AD is lacking. Objective: Our aim was to identify serum biomarker profiles in children with AD aged 0 to 17 years and compare these profiles with those previously found in adults with AD. Methods: Luminex multiplex immunoassays were used to measure 145 biomarkers in serum from 240 children with AD (aged 0-17 years). Principal components analysis followed by unsupervised k-means clustering were performed to identify patient clusters. Patients were stratified into age groups (0-4 years, 5-11 years, and 12-17 years) to assess association between age and cluster membership. Results: Children aged 0 to 4 years had the highest levels of T(H)1 cell-skewing markers and lowest levels of T(H)17 cell-related markers. T(H)2 cell-related markers did not differ significantly between age groups. Similar to the pattern in adults, cluster analysis identified 4 distinct pediatric patient clusters (T(H)2 cell/ retinol-dominant, skin-homing-dominant, T(H)1 cell/T(H)2 cell/ T(H)17 cell/HL-1-dominant, and T(H)1 cell/IL-1/eosinophil-inferior clusters). Only the T(H)1 cell/T(H)2 cell/T(H)17 cell/IL-1-dominant cluster resembled 1 of the previously identified adult clusters. Although no association with age or age of onset seemed to be found, disease severity was significantly associated with the skinhoming-dominant cluster. Conclusion: Four distinct patient clusters based on serum biomarker profiles could be identified in a large cohort of pediatric patients with AD, of which 1 was similar to previously identified adult clusters. The identification of endotypes driven by distinct underlying immunopathologic pathways might be useful to define pediatric patients with AD who are at risk of persistent disease and may necessitate different targeted treatment approaches.

【 授权许可】

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