Augmentation of intramembranous bone in rabbit calvaria using an occlusive barrier in combination with demineralized bone matrix (DBM): A pilot study

INTERNATIONAL JOURNAL OF SURGERY

卷:12

Article

Beltran, Victor^1,2  Engelke, Wilfried¹  Prieto, Ruth³  Valdivia-Gandur, Ivan⁴  Navarro, Pablo⁵  Cristina Manzanares, Maria⁶  Borie, Eduardo^2,7  Fuentes, Ramon² 

[1] Univ Gottingen, Dept Maxillofacial Surg, Gottingen, Germany

[2] Univ La Frontera, Fac Odontol, Dept Integral Adulto, Temuco 4781176, Chile

[3] Univ La Frontera, Fac Med, Temuco 4781176, Chile

[4] Univ Antofagasta, Dept Biomed, Unidad Anat Humana, Antofagasta, Chile

[5] Univ La Frontera, Fac Ingn, Dept Matemat & Estadist, Temuco 4781176, Chile

[6] Univ Barcelona, Unidad Anat & Embriol Humana, E-08007 Barcelona, Spain

[7] Univ Sao Paulo, Ribeirao Preto Dent Sch, BR-14049 Ribeirao Preto, Brazil

关键词: Bone regeneration;    Biomaterials;    DBM;    Barriers;    Bone;   

DOI  :  10.1016/j.ijsu.2014.03.010

来源: Elsevier

【 摘 要 】

The aim of this study was to histologically evaluate the performance of demineralized bone matrix (DBM) when compared with a blood clot in addition to an occlusive barrier in the bone regeneration process for bone defects in a rabbit model. Prefabricated metallic capsules with 4.5 mm and 3.5 mm dimensions were placed in five adult rabbit skulls. At the right side, the capsule was filled with DBM, and the clot was located on the left side. The barriers were supplied with a 0.5 mm horizontal peripheral flap and a vertical edge, fitting tightly into a circular slit prepared by a trephine in the skull. After a healing period of three months, the animals were sacrificed, and the samples were prepared for histological and histomorphometric analyses after capsule removal. Trabecular and medullar bone percentages were calculated from the different areas of the newly formed bone inside the metallic barriers, and non-parametric statistical analysis was used to describe the findings. The results showed a complete filling of newly formed bone inside the capsules of both groups. Less mature bone tissue was observed in the upper third of all samples, and a higher trabecular area was observed in the samples with DBM. The use of barriers resulted in the augmentation of newly formed bone in a three-month period. However, a higher trabecular area was observed in the barriers filled with DBM. (C) 2014 Surgical Associates Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

Free