Classification of the glyphosate target enzyme (5-enolpyruvylshikimate-3-phosphate synthase) for assessing sensitivity of organisms to the herbicide

JOURNAL OF HAZARDOUS MATERIALS

卷:408

Article

Leino, Lyydia¹  Tall, Tuomas¹  Helander, Marjo¹  Saloniemi, Irma¹  Saikkonen, Kari²  Ruuskanen, Suvi¹  Puigbo, Pere^1,3,4 

[1] Univ Turku, Dept Biol, Turku, Finland

[2] Univ Turku, Biodivers Unit, Turku, Finland

[3] Eurecat Technol Ctr Catalonia, Nutr & Hlth Unit, Reus, Catalonia, Spain

[4] Rovira & Virgili Univ, Dept Biochem & Biotechnol, Tarragona, Catalonia, Spain

关键词: Shikimate pathway;    Epsps enzyme;    Glyphosate;    Herbicide;    Resistance;    Microbiome;    Bioinformatics resource;    Biomarkers;    Sensitivity;   

DOI  :  10.1016/j.jhazmat.2020.124556

来源: Elsevier

【 摘 要 】

Glyphosate is the most common broad-spectrum herbicide. It targets the key enzyme of the shikimate pathway, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), which synthesizes three essential aromatic amino acids (phenylalanine, tyrosine and tryptophan) in plants. Because the shikimate pathway is also found in many prokaryotes and fungi, the widespread use of glyphosate may have unsuspected impacts on the diversity and composition of microbial communities, including the human gut microbiome. Here, we introduce the first bioinformatics method to assess the potential sensitivity of organisms to glyphosate based on the type of EPSPS enzyme. We have precomputed a dataset of EPSPS sequences from thousands of species that will be an invaluable resource to advancing the research field. This novel methodology can classify sequences from nearly 90% of eukaryotes and >80% of prokaryotes. A conservative estimate from our results shows that 54% of species in the core human gut microbiome are sensitive to glyphosate.

【 授权许可】

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