Kinetics of 5‐enolpyruvylshikimate‐3‐phosphate synthase inhibition by glyphosate

FEBS Letters

Coggins, John R.¹  Boocock, Martin R.¹ 

[1] Department of Biochemistry, University of Glasgow, Glasgow, G12 8QQ, Scotland

关键词: Shikimate pathway;    Glyphosate;    Fosfomycin;    5-Enolpyruvylshikimate-3-phosphate;    Neurospora crassa;    Arom enzyme complex;    Bis-tris;    bis(2-hydroxyethyl)imino-tris-(hydroxymethyl)methane;    DAHP;    3-deoxy-D-arabino-heptulosonate-7-phosphate;    DHQ;    3-dehydroquinate;    EPSP;    5-enolpyruvylshikimate-3-phosphate;    FMN;    flavin mononucleotide;    G6PDH;    glucose-6-phosphate dehydrogenase (EC 1.1.1.49);    HK;    hexokinase (EC 2.7.1.1);    LDH;    lactate dehydrogenase (EC 1.1.1.27);    PEP;    phosphoenolpyruvate;    PK;    pyruvate kinase (EC 2.7.1.40);    UDPGlcNAc;    uridine-5′-diphospho-N-acetyl-2-amino-2-deoxyglucose;   

DOI  :  10.1016/0014-5793(83)80888-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The herbicide glyphosate (N-phosphonomethy glycine) is a potent reversible inhibitor of the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase activity of the purified arom multienzyme complex from Neurospora crassa. Inhibition of the EPSP synthase reaction by glyphosate is competitive with respect to phosphoenolpyruvate, with K _i 1.1 μM, and uncompetitive with respect to shikimate-3-phosphate. The kinetic patterns are consistent with a compulsory order sequential mechanism in which either PEP or glyphosate can bind to an enzyme: shikimate-3-phosphate complex.

【 授权许可】

Unknown