The purification of 5‐enolpyruvylshikimate 3‐phosphate synthase from an overproducing strain of Escherichia coli

FEBS Letters

Coggins, John R.¹  Lewendon, Ann¹  Duncan, Kenneth¹ 

[1] Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland

关键词: 5-Enolpyruvylshikimate 3-phosphate synthase;    3-Phosphoshikimate 1-carboxyvinyltransferase;    aroA gene;    Escherichia coli;    Shikimate pathway;    Glyphosate;    Bis-tris;    bis(2-hydroxyethyl) iminotris-(hydroxymethyl)methane;    EPSP;    5-enolpyruvylshikimate 3-phosphate;    PEP;    phosphoenolpyruvate;    SDS;    sodium dodecyl sulphate;    shik 3-P;    shikimate 3-phosphate;   

DOI  :  10.1016/0014-5793(84)80027-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Escherichia coli aroA gene which codes for the enzyme 5-enolpyruvylshikimate 3-phosphate synthase (EPSP synthase) has been cloned from the λ-transducing bacteriophage λpserC. The gene has been located on a 4.7 kilobase pair PstI DNA fragment which has been inserted into the multiple copy plasmid pAT153. E. coli cells transformed with this recombinant plasmid overproduce EPSP synthase 100-fold. A simple method for the purification of homogeneous enzyme in milligram quantities has been devised. The resulting enzyme is indistinguishable from enzyme isolated from untransformed E. coli.

【 授权许可】

Unknown