Construction of a Genetic Multiplexer to Toggle between Chemosensory Pathways in Escherichia coli

JOURNAL OF MOLECULAR BIOLOGY

卷:406

Article

Moon, Tae Seok¹  Clarke, Elizabeth J.²  Groban, Eli S.²  Tamsir, Alvin³  Clark, Ryan M.⁴  Eames, Matthew²  Kortemme, Tanja^2,5  Voigt, Christopher A.^1,2,3 

[1] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Grad Grp Biophys, San Francisco, CA 94158 USA

[3] Univ Calif San Francisco, Tetrad Program, San Francisco, CA 94158 USA

[4] City Arts & Technol High Sch, San Francisco, CA 94112 USA

[5] Univ Calif San Francisco, Dept Biopharmaceut Sci, San Francisco, CA 94158 USA

关键词: genetic memory;    recombinase;    stochastic switching;    synthetic biology;    systems biology;   

DOI  :  10.1016/j.jmb.2010.12.019

来源: Elsevier

【 摘 要 】

Many applications require cells to switch between discrete phenotypic states. Here, we harness the FimBE inversion switch to flip a promoter, allowing expression to be toggled between two genes oriented in opposite directions. The response characteristics of the switch are characterized using two-color cytometry. This switch is used to toggle between orthogonal chemosensory pathways by controlling the expression of CheW and CheW*, which interact with the Tar (aspartate) and Tsr* (serine) chemoreceptors, respectively. CheW* and Tsr* each contain a mutation at their protein protein interface such that they interact with each other. The complete genetic program containing an arabinose-inducible FimE controlling CheW/CheW* (and constitutively expressed tar / tsr*) is transformed into an Escherichia coli strain lacking all native chemoreceptors. This program enables bacteria to swim toward serine or aspartate in the absence or in the presence of arabinose, respectively. Thus, the program functions as a multiplexer with arabinose as the selector. This demonstrates the ability of synthetic genetic circuits to connect to a natural signaling network to switch between phenotypes. (C) 2010 Elsevier Ltd. All rights reserved.

【 授权许可】

Free