A comprehensive appraisal of mechanism of anti-CRISPR proteins: an advanced genome editor to amend the CRISPR gene editing

Frontiers in Plant Science

Plant Science

Naveen Dhingra¹  Tathagata Ghosh²  Mahima Choudhary³  Virendra Kumar Yadav³  Dipty Tandi³  Nisha Choudhary³  Rakesh Kumar Verma³  Rajarshi K. Gaur⁴  Magda H. Abdellatif⁵  Byong-Hun Jeon⁶  Lienda Bashier Eltayeb⁷  Amel Gacem⁸  Krishna Kumar Yadav⁹  Mohammed S. Alqahtani^1,10 

[1] Department of Agriculture, Medi-Caps University, Indore, Madhya Pradesh, India;Department of Arts, School of Liberal Arts and Sciences, Mody University of Science and Technology, Lakshmangarh, Rajasthan, India;Department of Biosciences, School of Liberal Arts and Sciences, Mody University of Science and Technology, Lakshmangarh, Rajasthan, India;Department of Biotechnology, Deen Dayal Upadhyaya (D.D.U.) Gorakhpur University, Gorakhpur, Uttar Pradesh, India;Department of Chemistry, College of Sciences, Taif University, Taif, Saudi Arabia;Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, Republic of Korea;Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin AbdulAziz University-Al-Kharj, Riyadh, Saudi Arabia;Department of Physics, Faculty of Sciences, University 20 Août 1955, Skikda, Algeria;Faculty of Science and Technology, Madhyanchal Professional University, Ratibad, India;Environmental and Atmospheric Sciences Research Group, Scientific Research Center, Al-Ayen University, Thi-Qar, Nasiriyah, Iraq;Radiological Sciences Department, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia;Research Center for Advanced Materials Sciences (RCAMS), King Khalid University, Abha, Saudi Arabia;

关键词: anti-CRISPR;    genome editing;    Acr inhibitors;    genetic memory;    CRISPR-Cas complex;    DNA binding inhibition;    DNA cleavage;   

DOI  :  10.3389/fpls.2023.1164461

 received in 2023-02-12, accepted in 2023-05-23,  发布年份 2023

来源: Frontiers

【 摘 要 】

The development of precise and controlled CRISPR-Cas tools has been made possible by the discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs). The Acr protein has the ability to control off-targeted mutations and impede Cas protein–editing operations. Acr can help with selective breeding, which could help plants and animals improve their valuable features. In this review, the Acr protein–based inhibitory mechanisms that have been adopted by several Acrs, such as (a) the interruption of CRISPR-Cas complex assembly, (b) interference with target DNA binding, (c) blocking of target DNA/RNA cleavage, and (d) enzymatic modification or degradation of signalling molecules, were discussed. In addition, this review emphasizes the applications of Acr proteins in the plant research.

【 授权许可】

Unknown   
Copyright © 2023 Choudhary, Tandi, Verma, Yadav, Dhingra, Ghosh, Choudhary, Gaur, Abdellatif, Gacem, Eltayeb, Alqahtani, Yadav and Jeon