Suppression of progressive loss of coat color in microphthalmia-vitiligo mutant mice

JOURNAL OF INVESTIGATIVE DERMATOLOGY

卷:125

Article

Kurita, K ; Nishito, M ; Shimogaki, H ; Takada, K ; Yamazaki, H ; Kunisada, T

关键词: hair;    melanocyte;    microphthalmia-associated transcription factor;    pigmentation disease;    vitiligo;   

DOI  :  10.1111/j.0022-202X.2005.23861.x

来源: Elsevier

【 摘 要 】

The coat color of C57BL/6-Mitf(vit/vit) mice whitens with age, because of a one-nucleotide mutation in the DNA-binding region of the microphthalmia-associated transcription factor (MITF), which plays an important role in melanocyte growth and differentiation. To investigate the signals regulating MITF function, we prepared transgenic mice expressing three of the external signals that are important for melanocyte development, i.e., hepatocyte growth factor (HGF), stem cell factor (SCF), and endothelin-3 (ET3), and crossed these mice with Mitf(vit/vit) mice. We found that the age-dependent coat color whitening of the Mitf(vit/vit) mice was completely suppressed by the overexpression of HGF or SCF in the skin, but not by that of ET3. Moreover, HGF, but not ET3, promoted the proliferation of Mitf(vit/vit) mice-derived melanocytes in culture. These results suggest that the signals from exogenous HGF and SCF rescued the mi-vitiligo mutation and also that ET3 does not stimulate the common signal transduction pathway for MITF activation shared by HGF and SCF.

【 授权许可】

Free