C/T conversion alters interleukin-1A promoter function in a human astrocyte cell line

LIFE SCIENCES

卷:80

Article

Wei, Xing ; Chen, Xianming ; Fontanilla, Christine ; Zhao, Liming ; Liang, Zhong ; Dodel, Richard ; Hampel, Hampel ; Farlow, Martin ; Du, Yansheng

关键词: Alzheimer's disease;    interleukin-1 alpha;    neuroinflammation;    neurodegeneration;    promoter polymorphism;    A-beta;    LPS;   

DOI  :  10.1016/j.lfs.2006.12.011

来源: Elsevier

【 摘 要 】

Recently, association of an interleukin-1A promoter polymorphism (-889, thymine/thymine (T/T)) with Alzheimer's disease was reported, suggesting that this cytokine may play an important role in disease development. To understand the mechanism underlying the interleukin-1A promoter's role in Alzheimer's disease, a study comparing promoter function of an interleukin-1A polymorphism was performed in the SVG astroglia cell line. The effects of thymine and cytosine on transcriptional activity of the interleukin-1A promoter were analyzed by testing luciferase-reporter activity in transfected SVG cells. Our results demonstrate that cytosine/thymine conversion increases activity of the interleukin-1A promoter in SVG cells. Both sodium salicylate and lovastatin are able to block induced promoter activities in astroglial cells. Induced promoter activity by the polymorphism (T/T) may result in the upregulation of interleukin-1 alpha protein and cytokine cycle amplification, which may promote disease development. (c) 2007 Elsevier Inc. All rights reserved.

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