Rod-like particles of silica-coated maghemite: Synthesis via akaganeite, characterization and biological properties

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS

卷:476

Article

Veverka, Pavel¹  Pashchenko, Mariia¹  Kubickova, Lenka^1,2  Kulickova, Jarmila¹  Jirak, Zdenek¹  Havelek, Radim³  Kralovec, Karel^3,4  Kohout, Jaroslav²  Kaman, Ondrej¹ 

[1] Czech Acad Sci, Inst Phys, Cukrovarnicka 10, Prague 16200 6, Czech Republic

[2] Charles Univ Prague, Fac Math & Phys, V Holesovickach 2, Prague 18000 8, Czech Republic

[3] Charles Univ Prague, Fac Med Hradec Kralove, Simkova 870, Hradec Kralove 50038, Czech Republic

[4] Univ Pardubice, Fac Chem Technol, Studentska 573, Pardubice 53210, Czech Republic

关键词: Magnetic nanoparticles;    Magnetic nanorods;    Solvothermal procedure;    Mossbauer spectroscopy;    Cytotoxicity;   

DOI  :  10.1016/j.jmmm.2018.12.037

来源: Elsevier

【 摘 要 】

A multistep procedure, employing akaganeite FeO(OH,Cl) as a precursor, was developed for the preparation of rod-like maghemite particles for medical applications. At first, akaganeite rods with length of several hundred nm and width of approximate to 85 nm were prepared under hydrothermal conditions and were subsequently coated with mesoporous silica. Such coating enabled to maintain the shape of rods during the following steps that involved structural transformation of akaganeite to maghemite, i.e. gamma-Fe2O3. Then the original protective coating was removed by alkaline leaching, bare maghemite rods were isolated, and their structure and ferrimagnetic order were characterized by X-ray diffraction, TEM inspection, Mossbauer spectroscopy and SQUID magnetometry. The magnetization of the bare maghemite rods, that were formed by elongated clusters of approximate to 10-20 nm crystallites, made 47.0 and 41.7 Am-2/kg in magnetic field of 3 T at 5 K and 300 K, respectively. The hysteresis loops of both the bare and coated products at 300 K and ZFC/FC studies showed that the maghemite particles were largely blocked at room temperature in spite of the small size of crystallites. Finally, the particles were equipped with standard silica coating for biological studies. An evaluation of cytotoxicity of this silica-coated product was performed on two cell lines, namely A549 and MCF-7. The viability of cells incubated with particles at the concentration of 0.10, 0.21 and 0.42 mmol(Fe)/L was determined after 24 h and 48 h of incubation, and the values normalized to the viability of negative control were generally higher than 95%. Moreover, the real-time monitoring of cell adhesion, proliferation, and cytotoxicity by an xCELLigence system during 72 h of the incubation with particles revealed only some decrease of the cell index for the MCF-7 cells at the high concentration.

【 授权许可】

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