A comparison of salivary testosterone measurement using immunoassays and tandem mass spectrometry

PSYCHONEUROENDOCRINOLOGY

卷:71

Article

Welker, Keith M.¹  Lassetter, Bethany²  Brandes, Cassandra M.³  Prasad, Smrithi³  Koop, Dennis R.⁴  Mehta, Pranjal H.³ 

[1] Univ Massachusetts, Boston, MA 02125 USA

[2] Univ Iowa, Iowa City, IA 52242 USA

[3] Univ Oregon, Eugene, OR 97403 USA

[4] Oregon Hlth & Sci Univ, Portland, OR 97239 USA

关键词: Salivary testosterone;    Immunoassays;    Liquid chromatography;    Mass spectrometry;    Measurement;   

DOI  :  10.1016/j.psyneuen.2016.05.022

来源: Elsevier

【 摘 要 】

Enzyme immunoassays (EIAs) are widely used to measure salivary testosterone. However, little is known about how accurately different EIAs assess testosterone, partially because estimates across various EIAs differ considerably. We compared testosterone concentrations across EIAs of three commonly used manufacturers (DRG International, Salimetrics, and IBL International) to liquid chromatography tandem mass spectrometry (LC-MS/MS). Relative to EIAs from Salimetrics and IBL International, EIAs supplied by DRG International provided the closest approximation to LC-MS/MS testosterone concentrations, followed closely by EIAs from Salimetrics, and then IBL. Additionally, EIAs tended to inflate estimates of lower testosterone concentrations in women. Examining our results and comparing them to existing data revealed that testosterone EIAs had decreased linear correspondence with LC-MS/MS in comparison to cortisol EIAs. Overall, this paper provides researchers with information to better measure testosterone in their research and more accurately compare testosterone measurements across different methods. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

Free