Growth profile of Aspergillus niger on red galangal rhizomes as shown by bioactive compound changes

13th Joint Conference on Chemistry

Ratnasari^1 ; Cahyono, Bambang^1 ; Suzery, Meiny^1 ; Aminin, Agustina L.N.^1

Department of Chemistry, Faculty of Science and Mathematics, Diponegoro University, Semarang, Indonesia^1

关键词: Aspergillus niger;    Bioactive compounds;    LCMS;    Liquid chromatography;    mass spectrometries;    Metabolite profiles;    red galangal rhizomes;    Total flavonoids;    Total phenolic content;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/509/1/012118/pdf
DOI  :  10.1088/1757-899X/509/1/012118

来源: IOP

【 摘 要 】

This study we evaluated the growth profile of A. niger in red galangal rhizomes. The fermentation of red galangal rhizome was monitored based on hyphae characteristics followed by extraction with ethanol and determination of the total phenolic using Folin-Ciocalteu method and flavonoids using AlCl3 method. The metabolite profile was studied using Liquid chromatography-mass spectrometry. The results showed that A. niger was able to grow optimally on the 14th day of fermentation. The total phenolic and total flavonoids increased significantly compared to un-fermented product. LCMS profiles also showed the changes in the metabolite arrangement of fermented product comparing to the unfermented extract. There is 4-o-methyl-gallic acid produced by fermentation, which contributed to the increase in total phenolic content.