Imaging mass spectrometry assists in the classification of diagnostically challenging atypical Spitzoid neoplasms

JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY

卷:75

Article

Lazova, Rossitza¹  Seeley, Erin H.³  Kutzner, Heinz⁴  Scolyer, Richard A.^5,6,7  Scott, Glynis^8,9  Cerroni, Lorenzo¹⁰  Fried, Isabella^10,11  Kozovska, Milena E.¹²  Rosenberg, Arlene S.^13,14  Prieto, Victor G.¹⁵  Shehata, Bahig M.^16,18  Durham, Megan M.¹⁷  Henry, Gina^16,18  Rodriguez-Peralto, Jose L.¹⁹  Riveiro-Falkenbach, Erica⁶  Schaefer, Jochen T.^20,21  Danialan, Richard²²  Fraitag, Sylvie²³  Vollenweider-Roten, Sonja²⁴  Sepehr, Alireza^25,26  Sangueza, Martin²⁷  Hijazi, Nouf²⁸  Corredoira, Yamile²⁹  Kowal, Rachel^8,9,30  Harris, Olga M.³¹  Bravo, Francisco³²  Boyd, Alan S.³³  Gueorguieva, Ralitza²  Caprioli, Richard M.³⁴ 

[1] Yale Univ, Sch Med, Dept Dermatol, New Haven, CT 06510 USA

[2] Yale Univ, Dept Biostat, Sch Publ Hlth, New Haven, CT USA

[3] Protea Biosci Inc, Morgantown, WV USA

[4] Dermatopathol Friedreichshafend, Sydney, NSW, Australia

[5] Melanoma Inst Australia, Sydney, NSW, Australia

[6] Univ Sydney, Sydney Med Sch, Sydney, NSW, Australia

[7] Royal Prince Alfred Hosp, Tissue Pathol & Diagnost Oncol, Sydney, NSW, Australia

[8] Univ Rochester, Dept Dermatol, Med Ctr, Rochester, NY 14627 USA

[9] Med Univ Graz, Sch Med & Dent, Graz, Austria

[10] Med Univ Graz, Dept Dermatol, Graz, Austria

[11] Kempf & Pfaltz Histol Diagnost, Zurich, Switzerland

[12] Rabkin Dermatopathol Lab, Tarentum, Italy

[13] Case Western Reserve Univ, Dept Dermatol, MetroHlth Med Ctr, Cleveland, OH 44106 USA

[14] Cleveland Skin Pathology Lab Inc, Beachwood, OH USA

[15] MD Anderson Canc Ctr, Dept Pathol, Houston, TX USA

[16] Emory Univ, Sch Med, Dept Pathol, Atlanta, GA 30322 USA

[17] Emory Univ, Sch Med, Dept Surg, Atlanta, GA 30322 USA

[18] Univ Complutense Madrid, Facultad Medicina, Childrens Healthcare Atlanta, Madrid, Spain

[19] Univ Complutense Madrid, Facultad Medicina, Madrid, Spain

[20] Tufts Univ, Sch Med, Miraca Life Sci Newtons, Boston, MA 02111 USA

[21] Tufts Univ, Sch Med, Dept Dermatol, Boston, MA 02111 USA

[22] Tufts Med Ctr, Dept Pathol & Lab Med, Boston, MA USA

[23] Hop Necker Enfants Malad, Dept Pathol, Dermatopathol Praticien Hosp, Paris, France

[24] Viollier Morges SA Lausanne, Lausanne, Switzerland

[25] DermDX New England, Boston, MA USA

[26] Beacon Pathol, Dover, England

[27] Hosp Obrero Nro 1, Dept Pathol, La Paz, Bolivia

[28] Univ British Columbia, Dept Pathol, Vancouver, BC V5Z 1M9, Canada

[29] Univ Chile, Dept Pathol, Santiago, Chile

[30] Muhlbauer Dermatopathol Lab, Pittsford, NY USA

[31] Univ Fed Rio de Janeiro, BR-21941 Rio De Janeiro, Brazil

[32] Univ Peruana Cayetano Heredia, Dept Dermatol, Lima, Peru

[33] Vanderbilt Univ, Sch Med, Dept Dermatol, Nashville, TN 37212 USA

[34] Vanderbilt Univ, Sch Med, Mass Spectrometry Res Ctr, Nashville, TN 37212 USA

关键词: atypical Spitzoid neoplasm;    histopathology;    imaging mass spectrometry;    mass spectrometry;    Spitz nevus;    Spitzoid melanoma;   

DOI  :  10.1016/j.jaad.2016.07.007

来源: Elsevier

【 摘 要 】

Background: Previously, using imaging mass spectrometry (IMS), we discovered proteomic differences between Spitz nevi and Spitzoid melanomas. Objective: We sought to determine whether IMS can assist in the classification of diagnostically challenging atypical Spitzoid neoplasms (ASN), to compare and correlate the IMS and histopathological diagnoses with clinical behavior. Methods: We conducted a retrospective collaborative study involving centers from 11 countries and 11 US institutions analyzing 102 ASNs by IMS. Patients were divided into clinical groups 1 to 4 representing best to worst clinical behavior. The association among IMS findings, histopathological diagnoses, and clinical groups was assessed. Results: There was a strong association between a diagnosis of Spitzoid melanoma by IMS and lesions categorized as clinical groups 2, 3, and 4 (recurrence of disease, metastases, or death) compared with clinical group 1 (no recurrence or metastasis beyond a sentinel node) (P <.0001). Older age and greater tumor thickness were strongly associated with poorer outcome (P =.01). Conclusions: IMS diagnosis of ASN better predicted clinical outcome than histopathology. Diagnosis of Spitzoid melanoma by IMS was strongly associated with aggressive clinical behavior. IMS analysis using a proteomic signature may improve the diagnosis and prediction of outcome/risk stratification for patients with ASN.

【 授权许可】

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