Journal of Lipid Research | |
Spatial and temporal alterations of phospholipids determined by mass spectrometry during mouse embryo implantation[S] | |
Dale S. Cornett1  Stephen B. Milne1  Susanne Tranguch2  David S. Myers3  Kristin E. Burnum4  Satu M. Puolitaival4  Sudhansu K. Dey5  H. Alex Brown5  Richard M. Caprioli6  | |
[1] Departments of Biochemistry, Chemistry, Nashville, TN 37232;Departments of Biochemistry, Pharmacology, Nashville, TN 37232;Departments of Biochemistry, Chemistry, Nashville, TN 37232;Departments of Biochemistry, Mass Spectrometry Research Center, Nashville, TN 37232;Departments of Biochemistry, Pediatrics, Nashville, TN 37232;Departments of Biochemistry, Vanderbilt University Medical Center, Nashville, TN 37232; | |
关键词: imaging mass spectrometry; phosphatidylcholine; sphingomyelins; phosphatidylinositol; phosphatidylserine; phosphatidylglycerol; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Molecular events involved in successful embryo implantation are not well understood. In this study, we used MALDI imaging mass spectrometry (IMS) technologies to characterize the spatial and temporal distribution of phospholipid species associated with mouse embryo implantation. Molecular images showing phospholipid distribution within implantation sites changed markedly between distinct cellular areas during days 4–8 of pregnancy. For example, by day 8, linoleate- and docosahexaenoate-containing phospholipids localized to regions destined to undergo cell death, whereas oleate-containing phospholipids localized to angiogenic regions. Arachidonate-containing phospholipids showed different segregation patterns depending on the lipid class, revealing a strong correlation of phosphatidylethanolamines and phosphatidylinositols with cytosolic phospholipase A2α and cyclooxygenase-2 during embryo implantation. LC-ESI-MS/MS was used to validate MALDI IMS phospholipid distribution patterns. Overall, molecular images revealed the dynamic complexity of lipid distributions in early pregnancy, signifying the importance of complex interplay of lipid molecules in uterine biology and implantation.
【 授权许可】
Unknown