Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry

International Journal of Molecular Sciences

Nobuhiro Zaima¹  Takahiro Hayasaka²  Naoko Goto-Inoue² 

[1] Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan;

关键词: imaging mass spectrometry;    matrix-assisted laser desorption/ionization mass spectrometry;    biomarker;    protein;    lipids;   

DOI  :  10.3390/ijms11125040

来源: mdpi

【 摘 要 】

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland.