Exploring the biocatalytic potential of a DyP-type peroxidase by profiling the substrate acceptance of Thermobifida fusca DyP peroxidase

TETRAHEDRON

卷:72

Article; Proceedings Paper

Loncar, Nikola^1,2  Colpa, Dana I.²  Fraaije, Marco W.² 

[1] Univ Belgrade, Fac Chem, Studentski Trg 12-16, Belgrade, Serbia

[2] Univ Groningen, Mol Enzymol Grp, Nijenborgh 4, NL-9747 AG Groningen, Netherlands

关键词: Thermobifida fusca;    Biocatalysis;    Lignin;    Peroxidase;    Dye;    Vanillin;   

DOI  :  10.1016/j.tet.2015.12.078

来源: Elsevier

【 摘 要 】

Dye-decolorizing peroxidases (DyPs) represent a new class of oxidative enzymes for which the natural substrates are largely unknown. To explore the biocatalytic potential of a DyP, we have studied the substrate acceptance profile of a robust DyP peroxidase, a DyP from Thermobifida fusca (TfuDyP). While previous work established that this bacterial peroxidase is able to act on a few reactive dyes and aromatic sulfides, this work significantly expands its substrate scope towards lignin related compounds, flavors, and various dyes. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

Free