Final Technical Report

Wilson, David B.

关键词: BACTERIA;    CELLULASE;    CELLULOSE;    ENZYMES;    HYDROLYSIS;    MICROORGANISMS;    PROTEINS;    SOILS;    SYNTHESIS cellulase;    Thermobifida fusca;    regulation;   

DOI  :  10.2172/926446
RP-ID  :  DOEER13233-1
PID  :  OSTI ID: 926446
Others  :  TRN: US201123%%503

美国|英语

来源: SciTech Connect

【 摘 要 】

This grant provided the basic funding that enabled me to carry out a detailed characterization of the proteins used by the aerobic soil bacterium, Thermobifida fusca, to degrade cellulose and to study the mechanisms used by T. fusca to regulate cellulase synthesis. This work resulted in 53 publications and led to the decision by The DOE Joint Genome Institute to sequence the T. fusca genome. T. fusca is now recognized as one of the best studied cellulolytic microorganisms and our work led to the discovery of a novel class of cellulases, processive endoglucanases, which are found in many cellulolytic bacteria including both aerobes and anaerobes. In addition, we were able to determine the mechanism by which Cel9A caused processive hydrolysis of cellulose. This research also helped to explain why many cellulolytic microorganisms produce two different exocellulases, as we showed that these enzymes have different specificities, with one attacking the reducing end of a cellulose chain and the other attacking the nonreducing end. Our work also provided additional evidence for the importance of a cellulose binding domain (carbohydrate binding module) [CBM] in the hydrolysis of crystalline cellulose.