Non-enzymatic electrochemical determination of creatinine using a novel screen-printed microcell

TALANTA

卷:207

Article

Fava, Elson Luiz¹  do Prado, Thiago Martimiano¹  Garcia-Filho, Amauri¹  Silva, Tiago Almeida^1,2  Cincotto, Fernando Henrique^1,3  de Moraes, Fernando Cruz¹  Faria, Ronaldo Censi¹  Fatibello-Filho, Orlando¹ 

[1] Univ Fed Sao Carlos, Dept Chem, POB 676, BR-13560970 Sao Carlos, SP, Brazil

[2] Fed Ctr Technol Educ Minas Gerais, Dept Met & Chem, BR-35180008 Timoteo, MG, Brazil

[3] Univ Fed Rio de Janeiro, Inst Chem, Rio De Janeiro, RJ, Brazil

关键词: Screen-printed electrode;    Microcell;    Creatinine;    Iron (III);    Electrochemical sensor;   

DOI  :  10.1016/j.talanta.2019.120277

来源: Elsevier

【 摘 要 】

A low-cost and disposable microcell was constructed with a screen-printed electrode for the non-enzymatic electrochemical determination of creatinine. The working electrode was modified with carbon black and maintained in contact with paper-adsorbed iron (III) ions. A small sample volume of 3 mu L was required for the device operation. Then, iron (III) ions were complexed in the presence of creatinine in a chemical step, followed by an electrochemical reduction of non-complexed metallic ions in excess. Cyclic voltammetry and differential-pulse voltammetry experiments were employed for the electrochemical characterizations and analytical performance evaluation of the microcell. The working electrode modification with carbon black provided a significant increase of analytical signal. The sensor presented a linear response for creatinine concentrations ranging from 0.10 to 6.5 mmol L-1, with a limit of detection of 0.043 mmol L-1. Experiments for creatinine determination in real samples were successful performed through of standard recovery in urine.

【 授权许可】

Free