Microfluidic Device Directly Fabricated on Screen-Printed Electrodes for Ultrasensitive Electrochemical Sensing of PSA

Nanoscale Research Letters

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[1] 0000 0000 8653 0555, grid.203458.8, Department of Medical Imaging, Second Clinical College of Chongqing Medical University, No.74 Linjiang Road, Yuzhong District, 400016, Chongqing, China;0000 0001 0941 7177, grid.164295.d, Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, MD, USA;0000 0004 0368 8293, grid.16821.3c, Center of Food Safety Engineering and Technology Research, Shanghai, Key Laboratory of Urban Agriculture, Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, China;0000 0004 0368 8293, grid.16821.3c, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, 200030, Shanghai, China;0000 0004 0368 8293, grid.16821.3c, Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Key Lab. for Thin Film and Microfabrication Technology of Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, China;0000 0004 0368 8293, grid.16821.3c, Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, Key Lab. for Thin Film and Microfabrication Technology of Ministry of Education, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, China;0000 0004 0368 8293, grid.16821.3c, National Center for Translational Medicine, Collaborative Innovational Center for System Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, China;

关键词: Screen-printed electrode;    Microfluidic devices;    PSA;    Electrochemical sensor;    Detection;   

DOI  :  10.1186/s11671-019-2857-6

来源: publisher

【 摘 要 】

How to fabricate scale low-cost microfluidic device for detection of biomarkers owns a great requirement. Herein, it is for the first time reported that a new microfluidic device based on bonding polydimethylsiloxane microfluidic channels onto the substrate of a screen-printed electrode with coating glass solution was fabricated for electrochemical sensing of prostate-specific antigen (PSA). Compared to traditional microfabrication processes, this method is simple, fast, low cost, and also suitable for mass production. The prepared screen-printed electrode-based microfluidic device (CASPE-MFD) was used for the detection of the PSA in human serum. The prepared CASPE-MFD had a detection limit of 0.84 pg/mL (25.8 fM) and a good linearity with PSA concentration ranging from 0.001 to 10 ng/mL, which showed a great promise platform toward the development of miniaturized, low-cost electrochemical microfluidic device for use in human health, environmental monitoring, and other applications.

【 授权许可】

CC BY