An adhesive conducting electrode material based on commercial mesoporous titanium dioxide as a support for Horseradish peroxidase for bioelectrochemical applications

TALANTA

卷:146

Article

Rahemi, Vanoushe^1,2  Trashin, Stanislav¹  Meynen, Vera²  De Wael, Karolien¹ 

[1] Univ Antwerp, Dept Chem, AXES Res Grp, B-2020 Antwerp, Belgium

[2] Univ Antwerp, Dept Chem, Lab Adsorpt & Catalysis LADCA, B-2610 Antwerp, Belgium

关键词: Mesoporous titania;    Immobilized enzyme;    Hydrogen peroxide;    Composite electrode;    Amperometry;   

DOI  :  10.1016/j.talanta.2015.06.041

来源: Elsevier

【 摘 要 】

An adhesive conducting electrode material containing of graphite, biocompatible ion exchange polymer nafion (R) and commercial mesoporous TiO2 impregnated with horseradish peroxidase (HRP) is prepared and characterized by amperometric, UV-vis and N-2 sorption methods. The factors influencing the performance of the resulting biosensor are studied in detail. The optimal electrode material consists of 45% graphite, 50% impregnated HRP-TiO2 and 5% nafion (R). The optimum conditions for H2O2 reduction are an applied potential of -0.3 V and 0.1 mM hydroquinone. Sensitivity and limit of detection in the optimum conditions are 1 A M-1 cm(-2) and 1 mu M correspondingly. The N-2 sorption results show that the pore volume of TiO2 decreases sharply upon adsorption of HRP. The preparation process of the proposed enzyme electrode is straightforward and potentially can be used for preparation of carbon paste electrodes for bioelectrochemical detections. (C) 2015 Elsevier B.V. All rights reserved.

【 授权许可】

Free