Bacillus thuringiensis HCB6 Amylase Immobilization by Chitosan Beads

11th Joint Conference on Chemistry in Conjunction with the 4th Regional Biomaterials Scientific Meeting

材料科学;化学;生物科学

Zusfahair^1 ; Ningsih, D.R.^1 ; Kartika, D.^1 ; Fatoni, A.^1,2 ; Zuliana, A.L.^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Jenderal Soedirman, Purwokerto, Indonesia^1

Center for Maritime Biosciences Studies, Universitas Jenderal Soedirman, Purwokerto, Indonesia^2

关键词: Amylase activity;    Amylase production;    Bacillus thuringiensis;    Immobilization efficiency;    Immobilized enzyme;    Incubation temperatures;    Michaelis-Menten constant;    Substrate concentrations;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/172/1/012068/pdf
DOI  :  10.1088/1757-899X/172/1/012068

来源: IOP

【 摘 要 】

The purpose of this study was to optimize the amylase immobilization using a chitosan bead and to characterize immobilized amylase of Bacillus thuringiensis Bacteria HCB6. This study was started of amylase production, continued by immobilization optimization including ratio of chitosan:enzymes, enzyme-matrix contact time, substrate concentration, pH effect, incubation temperature effect, reaction time, and stability of immobilized enzyme. Amylase activity assay was dinitro salicylic (DNS) method. The results showed the optimum chitosan:enzyme ratio was 2.5: 1 (v/v), immobilization contact time of 18 hours and immobilization efficiency of 87.93%. Furthermore, immobilized amylase of B. thuringiensis HCB6 showed optimum substrate concentration of 1.5%, optimum pH of 6, optimum incubation temperature of 37 ° C, and the reaction time of 30 minutes. The Michaelis-Menten constant KMvalue for free and immobilized amylase were 5.30% and 1.33% respectively. Immobilized amylase can be used up to five times with the remaining activity of 43.3%.