Amylase production potentials of bacterial isolates obtained from the gut of Oryctes rhinoceros larvae

International Conference On Food Science and Engineering 2016

Aryati, P.C.^1 ; Pangastuti, A.^1 ; Sari, S.L.A.^1

Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Ir. Sutami street No. 36A Surakarta, Central Java

57126, Indonesia^1

关键词: Amylase activity;    Amylase production;    Amylolytic activity;    Bacillus Subtilis;    Bacterial isolates;    Pharmaceutical industry;    Residual activity;    Specific activity;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/193/1/012006/pdf
DOI  :  10.1088/1757-899X/193/1/012006

来源: IOP

【 摘 要 】

Amylase is one of the main enzymes used in industry, such as food, detergent, textile, and pharmaceutical industry. Amylase can be produced by plants, animals, and microorganisms. However, bacterial and fungal amylases have dominated application in industries. This research was aimed to determine amylolytic activity of bacteria isolated from the gut of Oryctes rhinoceros larvae. Based on clear zone formation, 9 from 11 isolates showed amylolytic activity. Isolates with the widest clear zone, i.e Bacillus subtilis GOR1, Bacillus cereus GOR3, and Bacillus pumilus GOR2, were screened for amylolytic activity based on reduction sugar production. The result showed that Bacillus subtilis GOR1 was the most potential as amylase producer, showed by the widest clear zone 5.224 cm2and highest reduction sugar production 0.0235 mg/ml. Highest amylase specific activity (0.1447 U/mg protein) was obtained at 60°C and pH 7. Amylase activity was stable for 3 hours at 60°C with residual activity respectively was 59.7%.