Laser ablation ICP-MS for simultaneous quantitative imaging of iron and ferroportin in hippocampus of human brain tissues with Alzheimer's disease

TALANTA

卷:197

Article

Cruz-Alonso, Maria¹  Fernandez, Beatriz¹  Navarro, Ana²  Junceda, Susana³  Astudillo, Aurora³  Pereiro, Rosario¹ 

[1] Univ Oviedo, Fac Chem, Dept Phys & Analyt Chem, Julian Claveria 8, E-33006 Oviedo, Spain

[2] Univ Oviedo, Fac Med, Dept Morphol & Cell Biol, Julian Claveria 8, E-33006 Oviedo, Spain

[3] HUCA, Serv Anatomopathol, Av Roma S-N, Oviedo 33011, Spain

关键词: Ferroportin;    Alzheimer's disease;    Iron accumulation;    Gold nanoclusters;    Quantitative bioimaging;    Laser ablation ICP-MS;   

DOI  :  10.1016/j.talanta.2019.01.056

来源: Elsevier

【 摘 要 】

Laser ablation inductively coupled plasma - mass spectrometry (LA-ICP-MS) is proposed for a better understanding of metals and proteins distribution in micrometre structures of human brain tissues. Simultaneous absolute quantitative imaging of Fe and ferroportin (FPN), in 5 urn thick tissue sections of the stratum pyramidale of hippocampus CA1 region, was carried out for Alzheimer disease (AD) patients and healthy controls (HC). For the imaging of FPN by LA-ICP-MS, antibodies were labelled via carbodiimide crosslinking with fluorescent gold nanoclusters (AuNCs) of 2.2 nm diameter, enabling a high amplification (314 gold atoms per NC). Laboratory made gelatin standards containing Fe and Au were used for LA-ICP-MS calibration. Results showed that iron presents an increased concentration in AD donors compared with HC donors, whereas similar concentrations of FPN in AD donors with respect to HC donors were obtained. The average absolute FPN concentrations in selected areas obtained with the proposed AuNCs method were compared with the levels obtained by densitometric analysis with a traditional IHC approach, observing a similar trend in all cases.

【 授权许可】

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