Efficient voltammetric discrimination of free bilirubin from uric acid and ascorbic acid by a CVD nanographite-based microelectrode

TALANTA

卷:130

Article

Taurino, Irene¹  Van Hoof, Viviane^2,3  Magrez, Arnaud⁴  Forro, Laszio⁵  De Micheli, Giovanni¹  Carrara, Sandro¹ 

[1] Ecole Polytech Fed Lausanne, Integrated Syst Lab, CH-1015 Lausanne, Switzerland

[2] Univ Antwerp Hosp, Lab Clin Biochem, Edegem, Belgium

[3] Univ Antwerp, Wilrtjk, Belgium

[4] Ecole Polytech Fed Lausanne, Crystal Growth Facil, CH-1015 Lausanne, Switzerland

[5] Ecole Polytech Fed Lausanne, Lab Phys Complex Matter, CH-1015 Lausanne, Switzerland

关键词: CVD nanographite;    Electrochemical biosensor;    Bilirubin;    Albumin;    Uric acid;    Ascorbic acid;    Human serum;   

DOI  :  10.1016/j.talanta.2014.07.009

来源: Elsevier

【 摘 要 】

We report a novel electrochemical sensor based on nanographite grown on platinum microelectrodes for the determination of bilirubin in the presence of normal concentrations of albumin. The albumin is a protein with an intrinsic ability to bind the bilirubin therefore reducing the concentration of the free electroactive metabolite in human fluids. In addition, the proposed device permits the discrimination of free bilirubin from two interferents, uric acid and ascorbic acid, by the separation of their oxidation peaks in voltammetry. Preliminary measurements in human serum prove that the proposed nanostructured platform can be used to detect bilirubin. (C) 2014 Elsevier B.V. All rights reserved.

【 授权许可】

Free