Preparation of hemoglobin-modified boron-doped diamond for acrylamide biosensors

International Symposium on Current Progress in Functional Materials

Umam, K.^1 ; Saepudin, E.^1 ; Ivandini, T.A.^1

Department of Chemistry, Faculty of Mathematics, Natural Sciences Universitas Indonesia, Kampus UI Depok, Depok

16424, Indonesia^1

关键词: Acrylamides;    Boron doped diamond;    Boron-doped diamond electrodes;    Concentration ranges;    Electrochemical behaviour;    Electrochemical biosensor;    Haemoglobins;    Sodium-acetate buffer;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/188/1/012006/pdf
DOI  :  10.1088/1757-899X/188/1/012006

来源: IOP

【 摘 要 】

Boron-doped diamond (BDD) electrode was modified with haemoglobin to develop electrochemical biosensors of acrylamide. Prior to modify with haemoglobin, the BDD was modified by gold nanoparticles to increase the affinity of BDD against haemoglobin. The electrochemical behaviour of the electrode in the presence of acrylamide was studied in comparison to haemoglobin-modified gold electrodes. Cyclic voltammetry indicated the optimum responses in 0.1 M sodium acetate buffer at pH 5. The responses were linear to the acrylamide concentration range of 5-50 μM with an estimated detection limit of 5.14 μM, suggesting that the electrode was promising for acrylamide biosensors.