BODIPY-derived piperazidine fluorescent near-neutral pH indicator and its bioimaging

SENSORS AND ACTUATORS B-CHEMICAL

卷:232

Article

Qin, Wenwu¹ 

[1] Lanzhou Univ, Coll Chem & Chem Engn, Key Lab Nonferrous Met Chem & Resources Utilizat, Lanzhou 730000, Peoples R China

关键词: BODIPY;    Piperazine;    Near-neutral pH;    Biological cells;   

DOI  :  10.1016/j.snb.2016.04.003

来源: Elsevier

【 摘 要 】

Two new 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) dyes (1 and 2) with piperazine subunits on position 3 or 5 were synthesized. Their absorption and steady-state fluorescence properties were investigated as a function of solvent. In water/MeCN (9:1, V/V) solution, the dyes show fluorescent enhancement upon increasing the acidity of the solution. They can be used in water/MeCN (9:1, V/V) solution as fluorescent pH probes excitable with visible light, with pK(a) values near-neutral (around 6.5 and 6.8). Dyes 1 and 2 are readily taken up by biological cells in the cytosol where it has no obvious toxic effects. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

Free