LIFTOSCOPE: development of an automated AI-based module for time-effective and contactless analysis and isolation of cells in microtiter plates

Journal of Biological Engineering

Research

Arnold Gillner¹  Nadine Nottrodt¹  Martin Wehner¹  Richard Lensing¹  Bastian Nießing²  Florian Narrog²  Tobias Piotrowski²  Niels König²  Robert H. Schmitt³ 

[1] Fraunhofer Institute for Laser Technology ILT, Aachen, Germany;Fraunhofer Institute for Production Technology IPT, Aachen, Germany;Fraunhofer Institute for Production Technology IPT, Aachen, Germany;WZL

[2]  RWTH Aachen University, Aachen, Germany;

关键词: Artificial intelligence (AI);    Biological cells;    Biomedical imaging;    High throughput cell isolation;    High-speed microscopy;    Laboratory automation;    Laser-induced forward transfer (LIFT);   

DOI  :  10.1186/s13036-023-00329-9

 received in 2022-05-18, accepted in 2023-01-27,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe cultivation, analysis, and isolation of single cells or cell cultures are fundamental to modern biological and medical processes. The novel LIFTOSCOPE technology aims to integrate analysis and isolation into one versatile, fully automated device.MethodsLIFTOSCOPE’s three core technologies are high-speed microscopy for rapid full-surface imaging of cell culture vessels, AI-based semantic segmentation of microscope images for localization and evaluation of cells, and laser-induced forward transfer (LIFT) for contact-free isolation of cells and cell clusters. LIFT transfers cells from a standard microtiter plate (MTP) across an air gap to a receiver plate, from where they can be further cultivated. The LIFT laser is integrated into the optical path of an inverse microscope, allowing to switch quickly between microscopic observation and cell transfer.ResultsTests of the individual process steps prove the feasibility of the concept. A prototype setup shows the compatibility of the microscope stage with the LIFT laser. A specifically designed MTP adapter to hold a receiver plate has been designed and successfully used for material transfers. A suitable AI algorithm has been found for cell selection.ConclusionLIFTOSCOPE speeds up cell cultivation and analysis with a target process time of 10 minutes, which can be achieved if the cell transfer is sped up using a more efficient path-finding algorithm. Some challenges remain, like finding a suitable cell transfer medium.SignificanceThe LIFTOSCOPE system can be used to extend existing cell cultivation systems and microscopes for fully automated biotechnological applications.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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