In situ observation of cellular organelles with a contact x-ray microscope

11th International Conference on X-ray Microscopy

Kado, M.^1 ; Kishimoto, M.^1 ; Tamotsu, S.^2 ; Yasuda, K.^2 ; Shinohara, K.^1,3

Japan Atomic Energy Agency, Umemidai, Kizugawa-shi, 619-0215, Kyoto, Japan^1

Nara Women's University, Kitauoyahigashi-machi, Nara-shi, 630-8506, Nara, Japan^2

Waseda University, Okubo, Shinjuku-ku, 169-8555, Tokyo, Japan^3

关键词: Biological cells;    Cellular organelles;    Gold target;    In-situ observations;    Inner structure;    Intense laser;    Single shots;    Spatial resolution;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/463/1/012056/pdf
DOI  :  10.1088/1742-6596/463/1/012056

来源: IOP

【 摘 要 】

A contact x-ray microscope coupled with a highly intense laser plasma soft x-ray source has been developed and in situ observations of cellular organelles have been conducted. The soft x-rays were generated by irradiating a high power laser pulse onto a thin-foiled gold target and about 1.3×1015photons/sr were obtained, which allowed the inner structures of live wet biological cells to be imaged. Single shot flash imaging is a key technique to image cellular organelles of live biological cells avoiding degradation of the spatial resolution of the images resulting from motion blur and radiation damage. The use of a fluorescence microscope to identify cellular organelles in conjunction with the soft x-ray microscope has allowed several cellular organelles to be identified precisely in the soft x-ray images. Combining the fluorescence microscope with the soft x-ray microscope will be very useful and will establish the technique as a powerful tool to analyze living function of biological cells.