On-chip electrocatalytic NO sensing using ruthenium oxide nanorods

SENSORS AND ACTUATORS B-CHEMICAL

卷:334

Article

Tanumihardja, E.¹  Rodriguez, A. Paradelo²  Loessberg-Zahl, J. T.¹  Mei, B.²  Olthuis, W.¹  van den Berg, A.¹ 

[1] Univ Twente, MESA Inst Nanotechnol, BIOS Lab Chip Grp, Enschede, Netherlands

[2] Univ Twente, Fac Sci & Technol, Photocatalyt Synth Grp, Enschede, Netherlands

关键词: Ruthenium oxide;    Electrocatalytic;    Nitric oxide;    Amperometric sensing;    Real-time mass-spectrometry;    Endothelial cells;   

DOI  :  10.1016/j.snb.2021.129631

来源: Elsevier

【 摘 要 】

Online, on-chip measurement of nitric oxide (NO) in organ-on-chip devices is desired to study endothelial (dys) function under dynamic conditions. In this work, ruthenium oxide (RuOx) is explored as an amperometric NO sensor and its suitability for organ-on-chip applications. For testing purposes, diethylamine NONOate was used as chemical NO donor. The NONOate's NO generation and electrochemical oxidation of generated NO were confirmed by real-time electrochemical/mass-spectrometry. Using RuOx nanorods electrodes, we show that NO oxidation occurred at a lower onset potential (+675 mV vs. Ag/AgCl) than on bare Pt electrode (+800 mV vs. Ag/AgCl). Due to NO adsorption on the RuOx surface, NO oxidation also delivered a higher current density (33.5 nA.mu M-1. cm(-2)) compared to bare Pt (19.6 nA.mu M-1. cm(-2)), making RuOx nanorods a favourable electrode for NO sensing applications. The RuOx electrode's suitability for organ-on-chip applications was successfully tested by using the electrode to detect a few micromolar concentration of NO generated by endothelial cell culture. Overall, the RuOx nanorods proved to be suitable for organ-on-chip studies due to their high sensitivity and selectivity. Our chip-integrated electrode allows for online NO monitoring in biologically relevant in vitro experiments.

【 授权许可】

Free