Grafting of antibodies inside integrated microfluidic-microoptic devices by means of automated microcontact printing

SENSORS AND ACTUATORS B-CHEMICAL

卷:140

Article

Chakra, Elie Bou²  Hannes, Benjamin²  Vieillard, Julien²  Mansfield, Colin D.²  Mazurczyk, Radoslav²  Bouchard, Aude³  Potempa, Jan^4,5  Krawczyk, Stanislas¹  Cabrera, Michel¹ 

[1] Univ Lyon 1, CNRS, UMR 5270, INL,ECL,INSA, F-69622 Villeurbanne, France

[2] Univ Lyon 1, CNRS, UMR 5270, INL,ECL,INSA,Ecole Cent Lyon, F-69134 Ecully, France

[3] IMEP, LAHC, UMR 5130, F-38016 Grenoble 1, France

[4] Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA

[5] Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Microbiol, Krakow, Poland

关键词: Microcontact printing;    Biochip;    Microfluidics;    Optical waveguide;    Antibody;   

DOI  :  10.1016/j.snb.2009.03.030

来源: Elsevier

【 摘 要 】

A novel approach to integrating biochip and microfluidic devices is reported in which microcontact printing is a key fabrication technique. The process is performed using an automated microcontact printer that has been developed as an application-specific tool. As proof-of-concept the instrument is used to consecutively and selectively graft patterns of antibodies at the bottom of a glass channel for use in microfluidic immunoassays. Importantly, feature collapse due to over compression of the PDMS stamp is avoided by fine control of the stamp's compression during contact. The precise alignment of biomolecules at the intersection of microfluidic channel and integrated optical waveguides has been achieved, with antigen detection performed via fluorescence excitation. Thus, it has been demonstrated that this technology permits sequential microcontact printing of isolated features consisting of functional biomolecules at any position along a microfluidic channel and also that it is possible to precisely align these features with existing components. (C) 2009 Published by Elsevier B.V.

【 授权许可】

Free