Portable cytometry using microscale electronic sensing

SENSORS AND ACTUATORS B-CHEMICAL

卷:224

Article

Emaminejad, Sam^1,2  Paik, Kee-Hyun²  Tabard-Cossa, Vincent³  Javanmard, Mehdi⁴ 

[1] Univ Calif Berkeley, Dept Elect Engn & Comp Sci, Berkeley, CA 94720 USA

[2] Stanford Univ, Sch Med, Stanford, CA 94305 USA

[3] Univ Ottawa, Dept Phys, Ottawa, ON K1N 6N5, Canada

[4] Rutgers State Univ, Dept Elect & Comp Engn, Piscataway, NJ 08855 USA

关键词: Impedance sensing;    Impedance cytometry;    Microfluidics;    Coulter counter;    Cytometer;   

DOI  :  10.1016/j.snb.2015.08.118

来源: Elsevier

【 摘 要 】

In this manuscript, we present three different micro-impedance sensing architectures for electronic counting of cells and beads. The first method of sensing is based on using an open circuit sensing electrode integrated in a micro-pore, which measures the shift in potential as a micron-sized particle passes through. Our micro-pore, based on a funnel shaped microchannel, was fabricated in PDMS and was bound covalently to a glass substrate patterned with a gold open circuit electrode. The amplification circuitry was integrated onto a battery-powered custom printed circuit board. The second method is based on a three electrode differential measurement, which opens up the potential of using signal processing techniques to increase signal to noise ratio post measurement. The third architecture uses a contactless sensing approach, which significantly minimizes the cost of the consumable component of the impedance cytometer. We demonstrated proof of concept for the three sensing architectures by measuring the detected signal due to the passage of micron-sized beads through the pore. (C) 2015 Elsevier B.V. All rights reserved.

【 授权许可】

Free